John Jay College, along with CUNY schools across the city, are moving toward the resumption of on-campus life. Classes are attended in-person, events are being planned, and professors and students alike are headed back into the laboratory. I talked to one of John Jay’s professors in the Department of Sciences, Dr. Jason Rauceo, to find out what it’s been like closing down and reopening his lab.

Dr. Rauceo is an Associate Professor of Biology whose research focuses on the major fungal pathogen Candida albicans. He is also the Director of the Cell and Molecular Biology major at John Jay. In 2021, Dr. Rauceo received a four-year grant from the National Institutes of Health to study the role of the mitochondrion in C. albicans’ ability to infect hosts and cause disease.

 

What was it like to get back to lab work after a significant time away during the early part of the pandemic? Did you have to change any protocols or make adjustments to your operating procedures?

Right before CUNY closed in March 2020, I shut the lab down with the expectation that I would not return for 6-12 months. I returned to campus in September 2020, and the major challenge was reopening the lab myself. Students were not allowed on campus, and I needed to calibrate several instruments that I had limited experience operating. Fortunately, my students were available via Zoom and FaceTime for assistance.

 

What does a typical day in the lab look like, if there is such a thing?

The day usually begins with a short one-to-one meeting with whomever is scheduled to perform an experiment, in which we mainly discuss logistics. Throughout the day, I periodically check in to assist and address any experimental issues if needed. At the end of the day, I inspect the lab to make sure that workspaces are cleaned, all reagents and supplies are properly stored, and all students have left the lab.

What function do students play in your lab?

Students perform the hands-on experimentation and data analysis, and are responsible for general lab maintenance. They also contribute to the development of their projects, which must be directly related to the lab agenda—in this case, C. albicans biology. Students may propose their own experiments for approval after approximately 1.5 to 2 years of experience in the lab.

 

You place a lot of emphasis on experiential-based learning. What does that mean in practice for your students?

I allow students to test their own hypotheses when safety and costs are not an issue. Also, I allow students to make their own errors during initial training exercises. I found that this approach in lab research builds confidence.

Generally, in the early stages of a new project, a significant amount of time is devoted to optimizing protocols to meet our objectives. During this “optimization phase” of the research, there is an extensive level of troubleshooting required, and a high level of error and ambiguity is observed. I found that a major payoff of experiential-based learning and training is that students propose unique approaches to addressing experimental obstacles.

 

Your study of SPFH (Stomatin, Prohibitin, Flotillin, HflK/HflC) proteins’ role in mitochondrial function in Candida albicans is being funded by the NIH. It seems that there are some exciting implications for developing antifungal treatments—can you tell me about that?

SPFH proteins are widely conserved in nature and are found in most living organisms. These proteins are important for major biological processes including, but not limited to, respiration, transport, and communication. Candida albicans is a fungus that resides in all humans on mucosal surfaces such as the mouth and gastrointestinal tract in a harmless state. However, changes in our immunity sometimes cause C. albicans infections. Immunocompromised individuals are highly susceptible to C. albicans infections.

Currently, the function of SPFH proteins is limited in C. albicans. We were the first research group to demonstrate that SPFH proteins are required when C. albicans is challenged with environmental stress. Our current proposal seeks to define the molecular function of SPFH proteins. We are collaborating with several prominent research groups in fungal biology and medicinal chemistry to determine the function of the SPFH proteins in mitochondrial function.

One of our project aims is to understand the effects of treating C. albicans with natural compounds that target SPFH proteins. Our initial findings are promising and may be useful in developing novel antifungal strategies.

The NIH award provided me with the funds to expand my lab operations, and I’ve recruited three new undergraduate students; therefore, I will be spending much of the next semester in student training and performing experiments.